Field: STRUCTURAL BIOLOGY
Contact person: Eva Pereiro, beamline responsible at MISTRAL
The achieved resolution of a 3D volume of a whole vitrified cell is presently limited by the optics. The use of Fresnel objective zone plates imposes a spatial resolution as well as a characteristic depth of field. In practice, the use of zone plates of 40 nm & 25 nm outermost-zone widths limits the depth of field to 3 µm & 1 µm, respectively, values quite below the typical thickness of an eukaryotic cell (10-20 µm).
This project aims at exploring new acquisition and computational methods to correct for the focusing limit imposed by the zone plates lenses, which decreases the 3D achievable spatial resolution in high-throughput full-field soft X-ray microscopes.
Collaborators: J Otón, JM Carazo (CNB-CSIC & Instruct Image Processing Center)
Funding: BioStruct-X 283570
