Soft X-ray Tomographic (SXT) microscopy is becoming a valuable technique for the analysis of the organization of cellular structures, filling a resolution gap between electron and confocal microscopy. SXT is based on the possibility of imaging three-dimensional fully hydrated cells under cryo conditions without any chemical pre-treatment or sectioning using soft X-rays. Unfortunately, SXT projections suffer from inaccuracies due to the limited depth of field (DOF) of the objective lens. Thus, modeling the image formation process is decisive to understanding how SXT projections are formed. We also analyze how the combination of multi-focal tomography mitigates the effect of these DOF inaccuracies.

For those who would like to attend the seminar from other institutions, please contact Dr. Eva Pereiro López to arrange access at the entrance gate.